Valutazione della firma interfonica di tipo II mediante realtime PCR One-Step Multiplex di tamponi faringei e campioni di sangue durante infezione da RSV nei bambini. ​

Globally, acute lower respiratory infection (ALRI) was one of the leading causes of morbidity and mortality in children younger than 5 years. The most common pathogen involved in ALRI is the Human Respiratory Syncytial virus (hRSV). In this study, we applied one-step real time rt-PCR technology type II INF signature to blood and nasopharyngeal (NP) swabs of acute early recovery children <1 years hospitalized for bronchiolitis with laboratory-confirmed RSV infection. The study was conducted in 2021-2022 and took place in Children Hospital “Regina Margherita”, in Torino, Italy. This study included 66 infants, of which 30 patients hospitalized for bronchiolitis, due to RSV infection, and 36 age matched controls. The IFN score, calculated as the fold change of the six genes for each patient, were significantly higher in healthy controls than in bronchiolitis subject in blood, but this data was not confirmed in nasopharyngeal swab. The discrepancy found between the blood and NP swab data is probably due to the nature of the biological sample, so this suggest that the NP swab reflects the real conditions of the subjects before the blood.