Innovative Nanoparticelle Proteiche: sintesi e caratterizzazione

Proteins represent an interesting material in formulation of nanoparticles for drug delivery. In general, protein nanoparticles compared to other NPs categories offer several advantages including biocompatibility and biodegradability. Moreover, Abraxane®, an albumin (HSA)-bound form of paclitaxel with a mean particle size of approximately 130nm, is an example of a clinical application of protein nanoparticles in delivery of anticancer drugs. The objective of the present study has been the development of a procedure for the preparation of [omissis:¿¿¿¿¿.] based nanoparticles. [omissis:¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿.¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿.]. Glycosylated nanostructures are promising carrier for drug delivery. In this case the materials can assume the endogenous display of carbohydrates on biological surfaces. Furthermore, carbohydrates on these surfaces cover an important role in the extracellular landscape because of their contribution to cell signaling, motility, adhesion and recognition. The possibility to bind carbohydrate-binding molecules as lectins to the saccharides on the surface of nanoparticles constitutes a relevant result for targeted therapies. The presence of saccharides on the nanoparticles surface and several factors of the preparation process, such as [omissis:¿¿¿¿¿¿¿...¿¿¿..] were evaluated. The obtained nanoparticles were characterized by UV¿vis spectrophotometry, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic light scattering (DLS) and zeta potential studies. The encapsulation efficiency and in vitro release of encapsulated fluorescein isothiocyanate from the [omissis:¿¿¿..] nanoparticles were evaluated. The cellular uptake of fluorescent nanoparticles was studied by confocal microscope imaging. Dye/Drug loaded [omissis:¿¿¿..] nanoparticles were synthetized and characterized as previously described. The interaction between [omissis:¿¿¿.] nanoparticles and lectin Concanavalin A was monitored through fluorescence spectroscopy studies.