Application of fluorescence in organoids: a guide to cellular complexity

Organoids have gained an increasing popularity in research due to their potential in addressing functional questions which might be poorly or not at all answered using classical 2D cell cultures. Their ability to recapitulate the architecture of a specific tissue, the mechanical and biochemical cues, the cell-cell and cell-ECM interactions makes them a promising avatar to investigate in vitro normal or pathological conditions. Yet this very complexity, which makes organoids such an attractive model, is challenging in terms of extraction of information. Imaging advances emerged from the last decade in addition to the use of fluorescent molecules are now allowing an even more informative view into these systems. The combination of these tools leads to better access to data contained within these models.