Vescicole di membrana derivate da probiotici come materiale di trasporto di farmaci per applicazioni di guarigione delle ferite

Wounds that do not follow the normal wound-healing progression may lead to chronic wounds, which burdens the patient and the health care system significantly. Today, conventional approaches, such as wound dressing and skin drafting, are used to treat chronic wounds. Innovative treatments, such as polymers and drug-incorporated materials, have been explored to accelerate wound healing in recent years. However, there is still no single approach that can be effectively applied to all types of wounds. Here, we demonstrate the encapsulation of gentamicin and curcumin into L. plantarum-derived membrane vesicles (LPMVs) to elicit biological activities in the resulting drug delivery system. We have encapsulated curcumin and gentamicin into LPMVs at maximum encapsulation efficiencies of 99% and 11%, respectively. The drug delivery system between curcumin and LPMVs (cur-LPMVs) resulted in the improvement of the stability and solubility of curcumin in water, antioxidant activity, and antimicrobial activity against S. aureus at 10:1 and 13:1 curcumin-to-LPMVs mass ratio. On the other hand, the drug delivery system between gentamicin and LPMVs (GS-LPMVs) did not show much improvement compared with pure gentamicin. Our results demonstrate that LPMVs as a drug delivery material are better suited for hydrophobic than for hydrophilic compounds. The main finding of this study is LPMVs ability to stabilize curcumin while preserving its biological activity.